纽约警察局里一如既往的忙碌着，老干探杰克·莫斯利(布鲁斯·威利斯)虽当年勇猛过人尽破些要紧的大案，可如今日渐衰老的他却勇猛不敢当年。但“姜还是老的辣！”虽身手打斗大不如前，但机警过人、经验老道的他却总能化险为夷，依然可以在很多重要案件上发挥着巨大的作用。眼前，便有一件10非常棘手的案件发生，一个名叫埃迪·班克(莫斯·戴夫)的黑人小子无意间看到了一起罪恶事件的发生，从而成为这起案件的重要目击证人。如今更是成了匪首党羽们竞相追杀的目标。因此，警方决定由资历深厚的老警探杰克·莫斯利来负责在开庭那天将目击证人从警局的看守所护送到相距16个街区之远的法庭现场。 而此时闻风而来的黑社会党羽们早已是蠢蠢欲动，这伙亡命之徒正是想利用这漫长而短暂的16个街区之路程来劫持并干掉证人埃迪，从而帮助他们的老大在法庭上因罪证不足而逃脱法律的制裁。而“黑证人”埃迪·班克却是个天生调皮喜欢无事打屁且乐观开朗的小子。性格不羁的他对穿制服的人也是从来都没有什么好感可言，对眼前的这位古板的老警察亦是百般的不合作。相反，老警探杰克对这位给他带来麻烦不断的黑人小子更是反感异常，但是出于对职业的尽心尽责，杰克还是拼尽全力与活力强劲的众匪徒们展开周折和惊险的斗智斗勇。而埃迪则似乎一点也没意识到将要面临怎样的险恶情境。在复杂而纷繁、不大不小的十六个街区之内，一场证人争夺战，或者说是一次亡命大逃杀就这样轰轰烈烈地开始了……
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